Anti-Bone Morphogenetic Protein 3 (BMP-3, Bone Morphogenetic Protein 3A, BMP-3A, Osteogenin, BMP3A,

The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and -II receptors. Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein, synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and -13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation. BMP3 / Osteogenin/ BMP-3A is a 472aa protein in human (chr 4p14-q21), highly expressed in lung, ovary and small intestine, Its function is involved in the cartilage and bone formation. BMP-3B / GDF-10, a 478aa protein in human (Chr 10q11.22), belongs to a group of proteins that can induce endochondral bone formation in adult animals, it is closely related in sequence to BMP3 with 44% homology. The aa sequences of human and rat BMP-3b precursor proteins are 83% similar, whereas the mature proteins show 98% identity. BMP-3B is mainly expressed in femur, brain, lung, pancreas and testis. Source of Antigen, Antibodies, Protein and controls. , Applications:, Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Western Blot: 1-10ug/ml (ECL), Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.