Anti-Bone Morphogenetic Protein 13 (BMP13, CDMP2)

The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and -II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function., BMP activities are modulated through gene expression, protein processing and by interaction with antagonists. The interplay between BMPs and their antagonists such as noggin & chordin governs developmental and cellular processes as diverse as establishment of the embryonic dorsal-ventral axis, induction of neuronal tissue, and formation of joints in the skeletal system and the neurogenesis in the adult brain., Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and -13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation., BMP13 / CDMP-2 / GDF-6, a cartilage derived morphogenetic protein with 436aa sequence with a 120aa polypeptide mature chain, exists as homodimer subunits linked with a disulfide bond. This cartilage derived morphogenetic protein (CDMP) is predominantly expressed in long bones during human embryonic development. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.