Anti-Bone Morphogenetic Protein 1, NT (BMP-1, ProCollagen C-endopeptidase, PCP, mTld, Mammalian Toll

The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14, other members, such as BMP-3, -6, -7 and -13 may be involved in later stages of skeletal formation. BMP1 was first identified in osteogenic bone extracts. It is an extracellular zinc endopeptidase implicated in a variety of species. BMP1 is expressed at high levels in embryonic maternal deciduum, membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung. BMP1 is a shorter spliced variant of mammalian tolloid (mTld) with a 986aa sequence (chr 8p21), both of which cleave the C-propeptides of type I procollagen during the synthesis of extracellular matrix collagen fibrils. BMP-1 comprises a metalloproteinase domain, 3 CUB domains, and an EGF-like domain, which is located between the second and third CUB domains. It cleaves the C-terminal propeptides of procollagen I, II and III, induces cartilage and bone formation. It shows 7 alternatives spliced forms and ubiquitously expressed. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml , Western Blot: 1-5ug/ml using ECL., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.