Anti-BMPR-1A (Bone Morphogenetic Protein, Activin receptor-like kinase, ALK-3, BRK-1 and SKR5)

Bone Morphogenetic Proteins (BMPs) are members of the TGF-beta superfamily of cytokines that affect bone and cartilage formation1. Mature BMPs are 30-38kD proteins that assume a TGF beta-like cysteine knot configuration1. Most BMPs are homodimers, but bioactive natural heterodimers have also been reported1. BMPs are involved in embryogenesis and, morphogenesis of various tissues and organs. They create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. BMPs also regulate the growth, differentiation, chemotaxis, proliferation, and apoptosis of mesenchymal cells, epithelial cells,, hematopoietic cells, and neuronal cells, and are responsible for normal dorsal/ventral patterning. Two type I receptors have been characterized, BMPR-IA (also designated activin receptor-like kinase ALK-3, BRK-1 and SKR5), and BMPR-IB (also designated ALK-6 and SKR 6), that bind to bone morphogenetic proteins (BMP)-2, BMP-4, and osteogenic protein (OP)-1 (also designated BMP-7)2-5. Type I receptors involved in BMP signaling can independently bind the, various BMP family proteins in the absence of type II receptors. BMPR-IA and BMPR-IB are thought to mediate distinct effects on gene expression, cell differentiation, and morphogenesis in a dose dependent fashion 2,6. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Immunofluorescence: 2ug/ml, Immunocytochemistry: 2ug/ml, Flow Cytometry: 3-5ug *10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.