Anti-Bit1, ID (PTRH2, BIT1, PTH2, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of trans

Adhesion to extracellular matrix regulates cell survival via integrin engagement and cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. A role for Bit1 (Bcl-2 inhibitor of transcription 1) has been identified in this process. Bit1 is a mitochondrial protein released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. Bit1-AES complexes may switch off a survival-promoting gene transcription program controlled by TLE. Apoptosis of Bit1/AES transfected cells is inhibited when cells are permitted to attach to fibronectin through the alpha-beta integrin, suggesting that the contribution of the Bit1-AES pathway to anoikis is regulated by integrins. Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Immunohistochemistry: 1:10-50, Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.