Anti-beta2-Microglobulin, clone 3H2718

Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119aa, chromosome 15). B2M associates with heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applictions not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:20 10ul labels 10e6 cells in 100ul., Immunohistochemistry: Frozen, Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOM myeloma cell line. Recommended Secondary Reagents: I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo, Recommended Negative Controls: I1904-78Y IgG1, Murine Negative Control (For rat tissue), Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.