Anti-beta-Catenin

beta-Catenin is a transcriptional coactivator that is encoded by the CTNNB1 gene in humans. It is a 781 amino acid protein comprised of an N-terminal domain containing glycogen synthase kinase 3beta (GSK3beta) phosphorylation sites, a C-terminal transactivation domain, and a central domain spanning amino acid residues 138-664. The central domain consists of 12 armadillo repeats and is required for binding to cadherins, TCF/LEF transcription factors, and adenomatous polyposis coli (APC). beta-Catenin has roles in cell adhesion, canonical Wnt signaling, regulation of stem cells, embryonic development, and adult tissue homeostasis, among others. In the absence of Wnt, a complex consisting of axin, APC, GSK3beta, and casein kinase 1 (CK1), binds to and phosphorylates beta-catenin, targeting it for ubiquitination and proteosomal degradation. In the presence of Wnt, phosphorylation of beta-catenin is inhibited, allowing beta-catenin to translocate into the nucleus, where it interacts with TCF/LEF to activate expression of Wnt target genes. Activating mutations in CTNNB1 that stabilize beta-catenin have been associated with a variety of cancers, including hepatocellular and adrenocortical carcinomas, colorectal cancer, and pilomatricomas. Cayman's beta-Catenin Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Catenin (Cadherin-associated Protein), Beta 1, CTNNB1, EVR7, MRD19, NEDSDV. Immunogen: Synthetic peptide corresponding to the N-terminal region of human beta-catenin. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: IHC, WB. UniProt Accession #: P35222. Origin: Animal/Rabbit.