Anti-beta-Amyloid Precursor Protein, CT (APP)

Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer's disease patients and Downs's Syndrome patients. In Alzheimer's disease, this protein is broken down and releases the 12 amino acid beta-amyloid protein that is found in senile plaques and vessels. The amyloid precursor protein is currently investigationed to determine how this protein is broken down abnormally in Alzheimer's disease to give rise to the beta-amyloid protein which is present in senile plaques and vessels. Applications: Detection of APP and the C99 fragment by Western blo. Recommended Dilution: Western Blot: 1:500 -1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Murine brain lysate It is mouse, rat and human reactive. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.