Anti-BD3 (beta Defensin-3)

A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, BD3) was isolated from human lesional psoriatic scales and cloned from keratinocytes. BD3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomycin-resistant Enterococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of BD3. Tumor necrosis factor alpha and contact with bacteria were found to induce BD3 mRNA expression. BD3 therefore might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic ?brosis. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1:500-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.