Anti-Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regul

Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer) is a 25-30kD member of the BCL-2 family of proteins. It is widely expressed, and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK is 211 aa in length and contains three BCL-2 homology domains (aa74-88, 117-136 and 169-184), a Zn-binding region (aa160-166) and a C-terminal , transmembrane segment (aa188-205). Amino acids 67-94 mediate oligomerization of BAK. There are two potential isoform variants, one shows an alternate start site at Met96, while a second shows a deletion of aa46-66. Over amino acids 20-124, human BAK shares 76% aa identity with mouse BAK. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.