Anti-BAD (Bcl-2 Antagonist of Cell Death, BBC6, BCL2L8)

Bcl­2 antagonist of cell death (Bad) is a 23kD cytoplasmic protein in the Bcl­2 family. It functions as a pro­apoptotic molecule by dimerizing with and inhibiting the anti­apoptotic proteins Bcl­2 and Bcl­xL. Prosurvival signals trigger the phosphorylation of Bad on Ser115, disrupting its interaction with Bcl­2 and Bcl­xL and resulting in protection from apoptosis. Human Bad shares 75% aa sequence identity with mouse and rat Bad. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.