Anti-ARF, p19

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop (3). These studies and others display the importance of p19ARF in cell cycle control and tumor suppression. By Western blot, this antibody recognizes IVT murine p19ARF and a clear band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs (1). The antibody can be competed from recognizing p19ARF using the antigenic peptide at 10ug/ml. , Recommended Dilution: , Western Blot: 0.5-1ug/ml (ECL), Immunohistochemistry: Not determined, ELISA (indirect): 1:5,000