Anti-Aquaporin 2 (AQP2, AQP-CD, AQPCD, WCH-CD)

Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4). Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:400, Western Blot: 1:1000-4000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.