Anti-Apolipoprotein E (Apo E)

ApoE is a 35-40kD protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. Applications: Suitable for use in Western Blot, Immuno double-diffusion, Radial Immunodiffusion, and Rocket IEP. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1,000 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.