Anti-alpha1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (glycerol, BSA & a

a1Antitrypsin (a1AT), also known as a1Proteinase inhibitor , (a1PI), is the most abundant protease inhibitor in blood and a member of the SERPIN family of proteinase inhibitors. Serum levels are typically 1.3mg/ml (2.5uM). a1AT is an acute phase protein and concentrations can rise four-fold during inflammatory episodes or tissue injury. Low levels in circulation have been associated with pulmonary disease such as emphysema. a1AT is a single chain molecule (52kD) that is produced primarily in the liver and to a lesser extent by blood monocytes and intestinal epithelium. Based on association rates, the primary target enzyme for a1AT is believed to be neutrophil elastase. a1AT is a broad-spectrum inhibitor for many serine proteinases. The main role of a1AT in vivo is likely that of a "backup" inhibitor and proteinase scavenger in fluids and tissues. Although the association rates of a1AT with other enzymes are lower, the high concentration in plasma makes it an important inhibitor of activated Protein C, activated F.XI, thrombin and plasmin. Enzyme inhibition by a1AT occurs through proteolytic cleavage between Met358 and Ser359, which induces a conformational change in a1AT locking the enzyme into a stable, inactive 1:1 enzyme-inhibitor complex. Application: Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.