Anti-alpha-Fetoprotein (AFP), Biotin conjugated, clone 1.B.806

Alpha-Fetoprotein (AFP) is a sialic acid glycoprotein with a molecular weight of 70 kD that consists of 590 amino acid residues. It has a single polypeptide chain with three repeating domains and it is structurally related to albumin. In normal tissue, AFP antibody intensely stains fetal liver, gastrointestinal tract, and yolk sac. The amount of AFP in fetal liver decreases with advancing gestational stage. In the abnormal tissue, AFP is expressed in hepatocellular carcinoma and gonadal and extragonadal germ cell tumors, including yolk sac tumors. AFP is considered a marker for hepatocellular histogenesis, and AFP immunostaining is found in 12% to 50% of hepatomas. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilution to be determined by the researcher. Recommended Pair: Suitable for use as the detection or conjugate (label) antibody., Matched to F4100-20 as the capture or solid phase antibody. Affinity Constant: 5x10e10 L/mole, Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Source: Ascites, Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ,