Anti-AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)

Anti-AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A1059-62M.50 50 µg - -

3 - 19 business days*

774.00€
 
Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human... more
Product information "Anti-AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)"
Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human Agouti-Related Protein (rhAgRP), also known as the protein product of Agouti-related transcript (ART). AgRP specific IgG was purified by human AgRP affinity chromatography and then biotinylated. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Can be used as a detection antibody in a human AgRP ELISA in combination with the monoclonal capture antibody (Catalog# A1059-62G). Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained. To arrive at the optimal dose range for , this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4ng/ml. , Western Blot: 0.1-0.2ug/ml. The detection limit for rhAgRP is approximately 1ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: A1059-62M

Properties

Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: Biotin
Host: Goat
Reactivity: Human
Immunogen: Sf 21-derived, recombinant human Agouti-Related Protein.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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