Anti-ADAMTS 5 (Aggrecanase 2, A Disintigrin And Metalloproteinase with Thrombospondin-3 motif)

Anti-ADAMTS 5 (Aggrecanase 2, A Disintigrin And Metalloproteinase with Thrombospondin-3 motif)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A0859-61E4.100 100 µg - -

3 - 19 business days*

720.00€
 
ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as... more
Product information "Anti-ADAMTS 5 (Aggrecanase 2, A Disintigrin And Metalloproteinase with Thrombospondin-3 motif)"
ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-macroglobulin (3), aggrecan (4), and brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and recombinant human eumatoid arthritis. The purified recombinant human ADAMTS5 starts at the, N?terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of recombinant human ADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and recombinant human TIMP-3. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 25ug/ml using conditioned culture medium spiked with recombinant human ADAMTS5., Western Blot: 0.1ug/ml using recombinant human ADAMTS5 as sample, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: A0859-61E4

Properties

Application: ELISA, IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Goat
Reactivity: Human
Immunogen: NS0-derived, recombinant human ADAMTS5, aa262 -622.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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