Anti-ADAMTS 10 (a Metallopeptidase with thrombospondin type 1 motif, 10)

ADAMTS10 belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent metalloproteases. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. Mutations in the ADAMTS10 gene may cause autosomal recessive Weill-Marchesani syndrome. The amino acid sequence of human ADAMTS10 is 100%, 96% and 94% identical to that of chimpanzee, dog and mouse. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml. Detection limit is ~10ng/well, Western Blot: 1-2ug/ml. Detection limit is ~25ng/lane under non-reducing conditions, Immunoprecipitation: 25ug/ml from conditioned cell culture media , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.