Anti-Actin, clone 2Q1055

Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986, Lessard, 1988). Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunofluorescence: 1:500 using NIH/3T3 cells. Positive staining pattern reflects both membrane and cytolasmic staining., Immunocytochemistry: 10ug/ml using methanol fixed mouse 3T3 cells, Immunohistochemistry (Paraffin): 10ug/ml using paraffin-embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections, Western Blot: 1:5000 using HEK293 lysates, ELISA: strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.