Anti-Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Bindin

Acrp30 (adipocyte complement-related protein of 30 kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28 kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Acrp30 (mouse 247 aa, rat human 244 aa, chromosome 3q27) consists of a predicted NT-signal sequence 91-14 aa), followed by a 27-aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Acrp30 is proteolytically cleaved at 104 aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml (ECL)., ELISA: 1:5000-25,000. Coat plate with 10-100ng of control peptide/well., Optimal dilutions to be determined by the researcher. Control Peptide: A0725-03 , Molecular Weights:, Full length Acrp30: ~30kD., Recombinant Acrp30: ~37kD, gAcrp30:~16kD. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.