Anti-Aak1, NT (AP2-associated protein kinase 1, Adaptor-associated kinase 1, DKFZp686F03202, DKFZp68

Anti-Aak1, NT (AP2-associated protein kinase 1, Adaptor-associated kinase 1, DKFZp686F03202, DKFZp68
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A0001-95B.100 100 µg - -

3 - 19 business days*

511.00€
 
AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein... more
Product information "Anti-Aak1, NT (AP2-associated protein kinase 1, Adaptor-associated kinase 1, DKFZp686F03202, DKFZp68"
AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist, the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Rat Spleen Tissue Lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: EC=2.7.11.1
Supplier: United States Biological
Supplier-Nr: A0001-95B

Properties

Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide corresponding to 20aa from near the amino terminus of the human Aak1.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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