Anti-4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell, 4-1BB homolog, T-cell antigen ILA, T

4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF , receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes, , neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF , ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen , presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell , costimulatory signal to promote survival, proliferation, cytokine production, and effector , functions. On dendritic cells, 4-1BB ligation enhances cytokine production and , upregulates expression of B7-1 and B7-2 costimulatory molecules. Applications: Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 3-10ug/ml/10, ELISA: 0.5-1.0ug/ml, Western Blot: 0.1-0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.