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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
0004-01D.500 | 500 µg | - | - |
3 - 19 business days* |
1,189.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
4-1BB Ligand is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF9).... more
Product information "Anti-4-1BB Ligand (4-1BBL)"
4-1BB Ligand is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF9). It is expressed on activated antigen presenting cells (APCs) and on most T and B lymphoma cell lines. Soluble 4-1BB Ligand can be released from the cell surface following cellular activation via proteolytic cleavage. 4-1BB Ligand exists as a noncovalent homotrimer and interacts with 4-1BB (TNFRSF9) to provide T cell costimulatory signals and dendritic cell activation. Applications: Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions., Flow Cytometry: 25ug/ml. 10ul of the diluted solution labels 1-2.5 x 10e5 cells in a total reaction volume ? 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as Gt xRt IgG conjugated to a fluorochrome. , Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1.5ng/well., Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, Sf 21-derived, recombinant mouse 4-1BB Ligand extracellular domain. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: | United States Biological |
Supplier-Nr: | 0004-01D |
Properties
Application: | ELISA, FC, WB |
Antibody Type: | Monoclonal |
Clone: | 8H117 |
Conjugate: | No |
Host: | Rat |
Species reactivity: | mouse |
Immunogen: | Sf 21-derived rm4-1BB Ligand extracellular domain |
Format: | Affinity Purified |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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