Anti-25-hydroxy Vitamin D3 Rabbit Monoclonal Antibody (Clone RM3)

25-hydroxy Vitamin D3 is an intermediate in the biosynthesis of 1alpha,25-dihydroxy vitamin D3 (calcitriol, Cay-71820), a vitamin D receptor (VDR) agonist that has multiple functions, including stimulation of calcium absorption, insulin secretion, and cell proliferation and differentiation. It is produced from vitamin D3 (Cay-11792) by 25-hydroxylase/CYP2R1 and bound to vitamin D binding protein (DBP) and albumin in the liver, then secreted into the bloodstream where it is the major circulating form of vitamin D. 25-hydroxy Vitamin D3 is transported to the kidney where it is hydroxylated by 1alpha-hydroxylase/CYP27B1 or 24-hydroxylase/CYP24A1 to produce 1alpha,25-dihydroxy vitamin D3 or 24,25-dihydroxy vitamin D3, respectively. Conversion of 25-hydroxy vitamin D3 to 1alpha,25-dihydroxy vitamin D3 is stimulated by parathyroid hormone (PTH) and inhibited by calcium, phosphorus, or FGF23. Serum 25-hydroxy vitamin D3 levels have been widely used as a marker of vitamin D status. Increased serum 25-hydroxy vitamin D3 levels are associated with increased bone density in patients with osteoarthritis, as well as reduced risk of colorectal, breast, and prostate cancer, whereas decreased serum 25-hydroxy vitamin D3 levels have been found in children with nutritional rickets, a condition caused by vitamin D deficiency and characterized by reduced bone density. Cayman's 25-hydroxy Vitamin D3 Rabbit Monoclonal Antibody (Clone RM3) can be used for ELISA.Synonyms: Calcidiol, Calcifediol, 25(OH)D3, 25-OH Vitamin D3. Immunogen: 25-hydroxy Vitamin D3-BSA conjugate. Clone Designation: RM3. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA. Cross Reactivity: (+) 25-hydroxy Vitamin D3, (+) 1alpha,25-hydroxy Vitamin D3, (-) Vitamin D3, (-) Vitamin D2, (-) 25-hydroxy Vitamin D2, (-) 1alpha,25-hydroxy Vitamin D2.