Anti-14-3-3 alpha/beta

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and in?uence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly in?uenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function. Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1.0ug/ml, Western Blot: 2ug/ml , Immunofluorescence (IC): 2ug/ml See reference #1, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.