Anti-c-Myc (Myc, myc-1, Cellular myelocytomatosis oncogene, Myc2, Niard, Nird, Transcription factor

Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins. , , Applications:, Western Blot: This antibody can be used at 2.0ug/ml with the appropriate secondary reagents to detect human c-Myc. , Immunohistochemistry: This antibody will detect human c-Myc in cells and tissues. The working dilution is 5-15ug/ml., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human c-Myc. The detection limit for rhc-Myc is approximately 0.2 ng/well. , ChIP- 5ug/5x10e6 cells, Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.