Anti-GFP Nanobody Affinity Agarose

Coupled ligand: Anti-GFP nanobody. Beads: Sepharose 4B agarose with average particle size of 50 µm. Binding capacity: 1.2mg protein /ml agarose. Storage buffer: PBS with preservative and 50% glycerol, pH7.2. Storage conditions: -20? for 12 months. Background: GFP, a green fluorescent protein, is derived from Aequorea victoria multi tube luminous jellyfish and is composed of about 238 amino acids. It can stimulate green fluorescence from blue light to ultraviolet light. GFP is often used as a marker for recombinant expression of eukaryotic proteins. Anti-GFP Nanobody affinity gel is made by covalently coupling high-quality GFP nanobody with agarose gel. As the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of the antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of GFP tagged fusion proteins.