Anti-b-Galactosidase (E. coli) (Sepharose)

Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein. Applications: Suitable for use in immunoprecipitation and for affinity purification of fusion proteins containing the tag. The binding capacity of the column for the affinity purification of the fusion protein must also be evaluated for a given fusion protein. Other applicatins not tested. Recommended Dilutions: Immunoprecipitation: 25-50ul of gel volume (50-10ul of 1:1 suspension) per 500ug of the protein lysate. For recombinant proteins with high level of expression, the lysate amounts must be optimized., Optimal dilution to be determined by the researcher. Storage and Stability: May be stored at 4°C. Do not freeze. Aliquots are stable for at least 12 months.