Catalogs from AAT Bioquest

AssayWise Letters 12-1

Read the most recent AssayWise Letter by our partner AAT Bioquest! Learn more about loop-mediated isothermal amplification (LAMP), one of the newest methods of nucleic acid amplification. If you prefer cell biology, take a look at the articles about intercellular channels, focusing on Gap Junctions, and about the potential of 3D cell culture. And in the final part of the letter, you will find a comprehensive guide to cell health assays in flow cytometry. Released December 2023.

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AAT Bioquest Selection Guides

The products made by our partner AAT Bioquest speak for themselves, but sometimes selection can still be a challenge, even for relatively common experimental designs. To make the process easier, AAT has built multiple selection guides for common categories of labeling reagents, including relative brightness comparisons where relevant, and all downloadable and printable for user convenience. Selection guides are available for: Annexin V, Antibody and Protein Labeling, Calcein, DNA Dyes, Flow Cytometry, Live or Dead Kits and Phalloidin. Released August 2023.

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PE & APC Tandem Dyes

Tandem dyes are highly effective fluorophores that offer exceptional brightness, making them ideal for detecting populations with lower receptor densities in flow cytometry applications. They are composed of a donor dye and an acceptor dye that are covalently linked together to form a single molecule, allowing for an energy transfer cascade (FRET). AAT Bioquest has developed a series of PerCP, PE and APC tandem dyes optimized for the 488 nm, 561 nm, and 633 nm laser lines. These bright dyes provide superior population resolution of low-expression markers and allow for the distribution of fluorochromes across multiple lasers and a wider spectrum. This reduces spillover and simplifies panel design, making the process more efficient and effective for researchers. Released June 2023.

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AssayWise Letters 11-1

DNA extraction is a fundamental method in molecular biology, despite being developed unintentionally. In the latest AssayWise Letter by our partner AAT Bioquest, you will get an overview of DNA extraction methods together with a general protocol for DNA extraction and purification. The AWL also includes comprehensive information on the ReadiUseTM 6-Color human TBNK antibody kit, which is designed to provide both the percentages and absolute counts of the lymphocyte subsets T-cells, B-cells, and NK-cells, using gated laser lines commonly present in flow cytometers. On top, you will find a practical overview of quantitative PCR (qPCR). Released April 2022.

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AssayWise Letters 10-2

Uncover the full spectrum of possibilities in AAT Bioquest's AssayWise Letter 10-2! Discover the potential of the PorteliteTM fluorimetric quantitation kits that are designed to selectively quantify total nucleic acid, DNA (ssDNA, dsDNA), RNA, endotoxin and protein. Or explore the world of fluorescent probe technologies suitable for multicolor spectral flow cytometry. AAT Bioquest is the expert when it comes to fluorescent technologies. So, you might also take a look at their fluorescent tools for studying mitochondrial morphology and function, which are featured in this AWL. Released December 2021.

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AssayWise Letters 10-1

Are you looking for tools to unravel the secrets of DNA? Then stop searching. In AAT Bioquest's AssayWise Letter, you will learn more about low-cost, ultra-sensitive fluorescence detection of DNA by gel electrophoresis using environmentally benign GeliteTM DNA gel stain and discover enzymatic and chemical labeling strategies for oligonucleotides. Talking about chemistry: The AWL also features an article on sensitive identification of newly synthesized DNA in situ using click chemistry-based Bucculite. And if you have trouble with your PCR products, you may read the practical guide for the detection and analysis of PCR products. Released April 2021.

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AssayWise Letters 9-2

The pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represented the worst threat to human health in over a century. Read this AssayWise Letter by AAT Bioquest and learn more about the development and applications of Coronavirus protease-specific CovidyteTM and CovipyteTM peptide substrates. These substrates can be used for the research of possible protease inhibitors for SARS-CoV-2 antiviral therapies. You will also find information about CalbryteTM 520 AM, a fluorescent calcium indicator for long-term in vitro imaging, and about T lymphocyte immunophenotyping using multicolor flow cytometry. And if this is not enough, you may take a look at the practical guide for labeling antibodies. Released December 2020.

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AssayWise Letters 9-1

Interested in microbiology? Then take a look at AAT Bioquest's AssayWise Letter to learn more about the cell-permeable nucleic acid probe SYTO® 9 and its variabilities of labeling live bacterial cells. And discover MycoLightTM fluorophore alternatives for live bacterial labeling and viability assessment. Talking about imaging live cells: You will also find a study about RatioWorksTM pH 165, a dual excitation/emission fluorescence probe for imaging live cells. Or dive into the world of Flow Cytometry and explore AAT's therapeutic monoclonal antibody fluorescent labeling tools. The last part focuses on TransfectamineTM 5000, an efficient and reliable DNA delivery system. Released April 2020.

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AssayWise Letters 8-2

This AssayWise Letter features a study about a simple end-point calcium assay using a green fluorescent indicator from AAT Bioquest. In addition, you will learn more about the coenzyme nicotinamide adenine dinucleotide (NAD+), the molecular link between cellular metabolism, signaling, and transcription, and discover AAT's selection of colorimetric and fluorimetric AmpliteTM NAD(P) and NAD(P)H assay kits. Other topics are Power StyramideTM signal amplification, which represents a superior alternative to tyramide signal amplification, and an article about fluorescent detection of cell senescence for Flow Cytometry and imaging applications. Released December 2019.

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AssayWise Letters 8-1

Read this AssayWise Letter to learn more about the CytoCiteTM fluorometer, the first cloud-integrated, single channel instrument designed for rapid fluorescence quantitative analysis and assay development. The CytoCiteTM fluorometer can measure proteins, nucleic acids, bacteria and other small molecules with a high degree of sensitivity, accuracy and reproducibility. Or discover antibody labeling kits that use the BuccutiteTM conjugation technology. Explore the workflow of this technology to prepare high-performance PE, APC and tandem conjugates for Flow Cytometry. Released April 2019.

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Calcium Detection Probes & Assay Kits

Calcium ion (Ca2+) impacts nearly every aspect of cellular life. This catalog illustrates the portfolio of our partner AAT Bioquest of fluorescent and luminescent calcium detection reagents together with non-fluorescent compounds for measuring and manipulating intracellular and extracellular Ca2+. Discover calcium signaling molecules and chelators, fluorescent single wavelength and ratiometric calcium indicators, luminescent calcium indicators as well as cAMP and phosphodiesterase (PDE) assays. AAT also offers a range of live cell calcium assays and tools for the measurement of calcium in vitroUpdated December 2018.

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AssayWise Letters 7-2

Check out this AssayWise Letters edition to get insights into the world of nitric oxide (NO), flow cytometry and metabolism research. Explore novel fluorescence probes for sensitive detection of exogenous and endogenous NO in live cells or learn more in the practical guide for live cell cycle analysis in Flow Cytometry. Expand your knowledge about NAD+ metabolism, bacterial viability and intracellular pH measurement and see what AAT has to offer to support your work in these fields. Released December 2018.

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AssayWise Letters 7-1

It is indisputable that mitochondria, also called the cell’s powerhouse, are the primary producers of cellular energy via oxidative phosphorylation and lipid oxidation. In this AssayWise Letter by AAT Bioquest, you will discover novel products and tools for studying mitochondrial functionality. In addition this letter highlights luciferin and allophycocyanin products and gives a brief description of AAT's ReadiUseTM TCEP Removal Buffer. And if you are working on actin, you might be interested in Phalloidin-iFluorTM conjugates that are useful to visualize actin filaments and that are featured in this brochure. Released September 2018.

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Fluorescence Microscopy Imaging Organelles

Organelles are tiny, specialized subunits that perform specific cellular functions. Typically membrane-bound and sequestered within the cytoplasm of eukaryotic cells, these organelles are key components essential for gene expression, cell signaling, energy production and protein synthesis. Detection, visualization and cellular tracking of such organelles in fluorescence imaging applications are made possible with the aid of organelle-selective dyes.
Released July 2018.

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AssayWise Letters 6-2

The future of calcium detection is bright! Learn more about the next generation calcium indicators from the CalbryteTM series developed by our supplier AAT Bioquest to monitor intracellular calcium. It includes three novel calcium indicators: CalbryteTM 520, CalbryteTM 590 and CalbryteTM 630. Other topics are a new direct ELISA for cAMP without acetylation and the quantitative comparison of iFluorTM 488- and FITC-conjugated antibodies. There are also two guides on selecting the right ROS probe as well as on the use of PE and APC in Flow Cytometry. Released June 2018.

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AssayWise Letters 6-1

Crosslinkers are a family of compounds that can be used to join together two or more macromolecules, such as proteins. The modication of proteins through crosslinking techniques provides insight into complex protein interactions as well as interaction domains. Explore the advantages of the new BuccutiteTM crosslinking technology by AAT Bioquest in this edition of AssayWise Letters. If you are interested in second messengers, you should read the article about NAADP-AM, a new player in calcium signaling pathways. In addition, this brochure will give exciting insights into nucleic acid staining and the quantitative analysis of thiols and maleimides. Released March 2018.

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Reactive Oxygen Species (ROS) Detection

Reactive oxygen species (ROS) are chemically reactive species containing oxygen. Oxygen atom has two unpaired electrons in separate orbits in its outer electron shell. This electron structure makes oxygen susceptible to radical formation. The sequential reduction of oxygen through the addition of electrons leads to the formation of a number of ROS including superoxide, hydrogen peroxide, hydroxyl radical, hypochlorous acid, peroxynitrite anion and nitric oxide. Released April 2017.

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AssayWise Letters 5-2

Download this AssayWise Letter to find out more about a set secondary antibodies conjugated with iFluorTM released by AAT Bioquest that are designed for robust and sensitive immunoassays. Or explore the history of calcium indicators and get an overview about the development of Cal-520® AM. In addition, this AWL focuses on current tools for DNA and RNA quantification and deals with hydrogen peroxide and salt-sensitive hypertension. Finally, you will get a look at NAD-dependent SIRT1, which plays a role in modulating cellular metabolism. Released April 2017.

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AssayWise Letters 5-1

Discover AAT Bioquest's portfolio of Horseradish peroxidase detection probes and assay kits as well as the broad collection of fluorescent calcium indicators in this AssayWise Letter. Moreover, you will find information about the ReadiLinkTM antibody labeling and ReadiViewTM biotinylation technology. This AWL also presents AAT's calcein-based cell viability assays, TR-FRET assays and a selection of products for fluorescence live cell imaging. And if that is not enough for you, take a look at the tools for RNA detection and quantification developed by AAT. Released April 2017.

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Cell Apoptosis & Proliferation

Are you working on cells and cell viability? Then check out this brochure from our partner AAT Bioquest, which contains various tools for investigating apoptosis, cell proliferation as well as cell viability and cytotoxicity. Discover assays for caspase activity and caspase binding, mitochondrion membrane potential assay kits and cell cycle assays. Or take advantage of the different CytoTellTM dyes for your live cell experiments. For the analysis of cell viability, AAT offers cell membrane integrity assays, together with dyes to label the nuclei of live or dead cells. Updated April 2017.

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