PDE4D2, active human recombinant protein, N-terminal GST tag

Human PDE4D2 (GenBank Accession No. NM_001197221), amino acids 2-507(end) with N-terminal GST tag, MW=84 kDa, expressed in a Baculovirus infected Sf9 cell expression system. 1 unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C in a reaction buffer. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, 0.1 mg/mlBSA and 0.1 ng/µl PDE4D2 at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.