VDR (human) Reporter Assay Kit

VDR (human) Reporter Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
IB-IB00701 96 wells -

7 - 11 business days*

1,448.00€
IB-IB00701-32 96 wells (3 x 32 wells) -

7 - 11 business days*

1,536.00€
 
This nuclear receptor assay system utilizes proprietary human cells engineered to provide... more
Product information "VDR (human) Reporter Assay Kit"
This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO's reporter cells include the luciferase reporter gene functionally linked to a VDR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in VDR activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human VDR. VDR reporter cells are prepared using INDIGO's proprietary CryoMite(TM) process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or pre-incubation of reporter cells prior to assay setup. INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to VDR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
Keywords: VDR, NR1I1, Vitamin D3 receptor, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1
Supplier: Indigo Biosciences
Supplier-Nr: IB00701

Properties

Application: Functional activity screening
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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