PGR Reporter Assay System (dog)

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Dog Progesterone Receptor (dPGR). INDIGO's dPGR reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the dPGR. In addition to dPGR Beta Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against dPGR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture. Features Clear, Reproducible Results All-Inclusive Assay Systems Exceptional Cell Viability Post-Thaw Consistent Results Lot to Lot Product Specifications Target Type Nuclear Hormone Receptor, Nuclear Receptor Orthologs Species Dog Receptor Form Hybrid Assay Mode Agonist, Antagonist Kit Components Dog PGR Reporter Cells Cell Recovery Medium (CRM) Compound Screening Medium (CSM) Progesterone, 1 mM (in DMSO) Detection Substrate Detection Buffer White, sterile, cell-culture ready assay plate Shelf Life 6 months. Target Background: This nuclear receptor assay utilizes proprietary cells engineered to provide constitutive, high-level expression of the full-length Dog Progesterone Receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PR or PGR. The principal application of this assay is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the dog progesterone receptor. It is an all-inclusive assay system that includes, in addition to PGR Reporter Cells, two optimized media for use during cell culture and for preparing dilutions of test samples, the reference agonist Progesterone, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol. Protein function: The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor. [The UniProt Consortium]