Reporter Assays

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human thyroid hormone receptor beta (NR1A2), a ligand-dependent transcription factor commonly referred to as TRbeta. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the mouse peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or mPPARalpha. INDIGO's reporter cells include...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the rat peroxisome proliferator-activated receptor delta (NR1C2), a ligand-dependent transcription factor commonly referred to as PPARD or rPPARdelta. INDIGO's reporter cells include...

Protein function: Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific...

Prostaglandin F2alpha (PGF2alpha) is a biologically important prostanoid that exerts its actions through binding to the PGF2alpha receptor (FP). PGF2alpha is synthesized in many tissues, including the eye and organs of the female reproductive system. Activation of ocular FP receptors via agonists such as bimatoprost...

The family of Fibroblast Growth Factors (FGFs) comprise approximately 23 members that are related by core sequence and structure conservation, with the majority of FGFs being secreted signaling proteins. Secreted FGFs are predominantly autocrine and paracrine factors, with only three members evolved to function as...

INDIGlo Luciferase Detection Reagent (LDR) is formulated to deliver steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided 'ready to use', without the requirement to first re-constitute powder-form components. INDIGlo LDR works...

This Rat Estrogen Receptor Alpha (rERalpha) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to rERalpha Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a...

This Mouse Aryl Hydrocarbon Receptor (mAhR) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to mAhR Reporter Cells, two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist (MeBIO), Luciferase Detection Reagent, and...

This Estrogen-related Receptor Beta (ERRbeta) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to ERRbeta Reporter Cells, two optimized media for use during cell culture and in diluting the user's test samples, the reference inverse-agonist 4-Hydroxy Tamoxifen,...

This Estrogen-related Receptor Beta (ERRbeta) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to ERRbeta Reporter Cells, two optimized media for use during cell culture and in diluting the user's test samples, the reference inverse-agonist 4-Hydroxy Tamoxifen,...

The Orexin 1 Receptor (OX1R) may be an important therapeutic target for treatment of sleep disorders, obesity, emotional stress, and addiction. Cayman's OX1R Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for OX1R, a recombinant G-protein, and a cAMP...