Anti-phospho-MAPK (Thr202/Tyr204) (Mitogen Activated Protein Kinase, extracellular-signal regulat

MAPK is activated by phosphorylation, whereupon the protein translocates to the nucleus, where it phosphorylates nuclear targets. MAPK is an important integration point of many signalling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. The specificity of this antibody has been shown by blocking studies in Western blot, whereby staining was blocked by the phosphopeptide. No blocking was seen by the corresponding non-phosphopeptide. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1:1000, detects a band of approximately 43kD in human T47D lysates, Optimal dilutions determined by the researcher. Recommended Secondary Reagents: Sheep anti rabbit IgG:HRP , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.