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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
B5000-10.100 | 100 µl | - | - |
3 - 19 business days* |
757.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases.... more
Product information "Anti-phospho-BTK (Ser180) (Bruton's Tyrosine Kinase), clone 3i5"
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCbeta through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/Btk inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, For Western Blot, USBio recommends using immunoprecipitated Btk., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | EC=2.7.10.2, Anti-phospho-ATK, Anti-phospho-BPK, Anti-phospho-AGMX1, Anti-phospho-Bruton tyrosine kinase, Anti-phospho-B-cell progenitor kinase, Anti-phospho-Tyrosine-protein kinase BTK, Anti-phospho-Agammaglobulinaemia tyrosine kinase |
Supplier: | United States Biological |
Supplier-Nr: | B5000-10 |
Properties
Application: | ELISA, WB |
Antibody Type: | Monoclonal |
Clone: | 3i5(3D3) |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
MW: | 77.// kD |
Format: | Affinity Purified |
Database Information
KEGG ID : | K07370 | Matching products |
UniProt ID : | Q06187 | Matching products |
Gene ID | GeneID 695 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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