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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
U1000-07E.250 | 250 µg | - | - |
3 - 19 business days* |
644.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of... more
Product information "Anti-Ubiquitin (RPS27A, UBA52, UBB, UBC)"
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56kD which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome, a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a). Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (formalin-fixed, paraffin-embedded): 1:150 -1:300, ELISA: Indirect, Optimal dilutions to be determined by the researcher. Recommended Positive Control: Endometrial Tissue Homogenates, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-CEP52, Anti-UBCEP2, Anti-60S ribosomal protein L40, Anti-Ubiquitin-60S ribosomal protein L40, Anti-Ubiquitin A-52 residue ribosomal protein fusion product 1 |
Supplier: | United States Biological |
Supplier-Nr: | U1000-07E |
Properties
Application: | ELISA, IHC, IP, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | chicken, human, monkey, rat |
Immunogen: | Ubiquitin isolated from bovine erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K02927 | Matching products |
UniProt ID : | P62987 | Matching products |
Gene ID | GeneID 7311 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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