Anti-Tumor Necrosis Factor Receptor 1, p55/p60 (TNFR 1, TNFR1, TNF-R1, TNF-R, Tumor Necrosis Factor

Tumor necrosis factor receptor 1 (TNF Receptor 1) binds cytokines TNF-alpha and TNF-beta resulting in the activation of pathways involved in both cell survival and apoptosis. TNF Receptor 1 contains an extracellular death domain that binds to the death domain adaptor protein TRADD and triggers the activation of caspases. At least 3 isoforms are produced by alternative splicing. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 0.5-1ug/ml using formalin-fixed, paraffin-embedded tissue. Requires het mediated antigen retrieval., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.