Anti-Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, TOP2, TOP2A, TP2A)

Anti-Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, TOP2, TOP2A, TP2A)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
T8065-12G.100 100 µg - -

3 - 19 business days*

494.00€
 
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to... more
Product information "Anti-Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, TOP2, TOP2A, TP2A)"
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22.The TOP2A gene spans approximately 30kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1-2ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: T8065-12G

Properties

Application: WB
Antibody Type: Monoclonal
Clone: 9F296
Conjugate: No
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen.
Purity: Rabbit ascites
Format: Ascites

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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