Anti-TopBP1 (DNA Topoisomerase 2 Binding Protein 1, DNA Topoisomerase II Binding Protein, DNA Topois

TOP2B (DNA Topoisomerase II b) is a 180-185kD member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/a-form being 170kD in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relieves torsional stress created in DNA during transcription, or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626aa in length. It contains an ATPase domain (aa101-201), an NES (aa1034-1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa1508-1611). There is one splice variant that shows a deletion of (aa24-28). Over (aa1187-1621), human TOP2B shares 91% aa identity with mouse TOP2B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.