Anti-Thimet Oligopeptidase 1 (TOP1)

Thimet oligopeptidase (THOP1), also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase. Widely expressed by mammalian tissues and reported to be present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme capable of hydrolyzing a number of bioactive peptides and peptides released from by the proteosome, limiting antigenic presentation by MHC class I molecules. The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: : 1-2ug/ml with the appropriate secondary reagents to detect human THOP1. Using a colorimetric detection system, the detection limit for rhTHOP1 is approximately 50ng/lane under non-reducing and reducing conditions., Immunoprecipitation: 25ug/ml to immunoprecipitate rhTHOP1 from conditioned cell culture medium. Under similar conditions, this antibody did not immunoprecipitate rhNeurolysin., Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human THOP-1. The detection limit for rhTHOP-1 is approximately 3 ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.