Anti-SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S.

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:128,000, Western Blot: 0.1-0.3ug/ml ~28kD band observed in Human Liver lysates (calculated MW of 28.6kD according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kD and 48kD are observed. These bands are not observed in the non-transfected HEK293. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.