Anti-Serotonin (5-Hydroxytryptamine, 5HT)

Applications: , Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: >1:500 , Immunohistochemistry: 1:400-1:800 in PBS/0.3% Triton X-100, Cy3 technique, 1:5000-1:10,000 in PBS/0.3% Triton X-100, Biotin/Avidin-HRP, Optimal dilutions to be determined by the researcher. Immunohistochemistry Application Notes: Perfusion Fixation: Fixative- 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4, 500ml, over 20 minutes., Post Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4, 500ml, over 20 minutes., Post Fixation: 1.5 hour at 4°C. 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4., Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1- 0.3%) may be used in conjunction with paraformaldehyde., Sections: 10um cryostat or 50um vibratome, Absorption control: 5-HT/BSA 25ug/ml diluted serum. Incubation on Tissue: 16-24 hours at 4°C. Positive Control: Rat hypothalamus, raphe nuclei, spinal cord, Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.