Anti-Serotonin (5-Hydroxytryptamine, 5HT)

Anti-Serotonin (5-Hydroxytryptamine, 5HT)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S1001-09A.100 100 µl - -

4 - 22 business days

588.00€
 
Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not... more
Product information "Anti-Serotonin (5-Hydroxytryptamine, 5HT)"
Applications: , Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: >1:500 , Immunohistochemistry: 1:400-1:800 in PBS/0.3% Triton X-100, Cy3 technique, 1:5000-1:10,000 in PBS/0.3% Triton X-100, Biotin/Avidin-HRP, Optimal dilutions to be determined by the researcher. Immunohistochemistry Application Notes: Perfusion Fixation: Fixative- 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4, 500ml, over 20 minutes., Post Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4, 500ml, over 20 minutes., Post Fixation: 1.5 hour at 4°C. 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4., Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1- 0.3%) may be used in conjunction with paraformaldehyde., Sections: 10um cryostat or 50um vibratome, Absorption control: 5-HT/BSA 25ug/ml diluted serum. Incubation on Tissue: 16-24 hours at 4°C. Positive Control: Rat hypothalamus, raphe nuclei, spinal cord, Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: S1001-09A

Properties

Application: IHC, WB
Antibody Type: Polyclonal
Host: Goat
Reactivity: Human, Mouse, Rat
Immunogen: Whole molecule 5HT
Format: Serum

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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