Anti-S-phase Kinase-associated Protein 2 (SKP2, Cyclin-A/CDK2-associated Protein p45, F-box Protein

S-phase kinase-associated protein 2 (SKP2) is a subunit in the SKP1-CUL1-F-box protein E3 ubiquitin protein ligase complex. This complex mediates the ubiquitination and the subsequent degradation of proteins involved in cell cycle progression, signal transduction and transcription. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry (Paraffin and Formalin): 1.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 100ul sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.