Anti-RAD18 (hHR18, hRAD18, Postreplication Repair Protein hRAD18p, Postreplication Repair Protein RA

Anti-RAD18 (hHR18, hRAD18, Postreplication Repair Protein hRAD18p, Postreplication Repair Protein RA
Item number Size Datasheet Manual SDS Delivery time Quantity Price
R0445-25B.100 100 µg - -

3 - 19 business days

479.00€
 
Human Rad18 (hRad18) is homolog of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a... more
Product information "Anti-RAD18 (hHR18, hRAD18, Postreplication Repair Protein hRAD18p, Postreplication Repair Protein RA"
Human Rad18 (hRad18) is homolog of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56kD. The human Rad18 is ubiquitously expressed in various human tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: HL60 , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: R0445-25B

Properties

Application: WB
Antibody Type: Monoclonal
Clone: 9C19
Conjugate: No
Host: Mouse
Reactivity: Human
Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.
Purity: Purified by immunoaffinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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