Anti-Protein Kinase C, mu (PKCu)

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic, nucleotide-independent enzyme that phosphorylates serine and threonine residues. The PKC family is divided into three groups depending on each the enzymes' cofactor requirements: conventional (c)PKC isoforms (alpha, betaI, betaII, and gamma) require diacylglycerol (DAG), Ca2+, and phospholipids for activation, novel (n)PKC isoforms (delta, epsilon, eta (also known as PKC-L), theta, and µ the mouse homolog of human PKCµ (also known as PKD)) require DAG but not Ca2+ for activation, and atypical (a)PKC isoforms, (zeta, iota, and lambda, the mouse homolog of human PKC iota) require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCnu., PKC's phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. These kinases also serve as major receptors for phorbol esters, a class of tumor promoters. Every member of the PKC family has a specific expression profile and is believed to play a distinct role in cells., PKCµ, also known as nPKC-D1 and nPKC-mu, is a member of PKC family of serine/threonine kinases. PKCµ has two characteristic cysteine-rich repeats, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. , Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.