Anti-Profilin, Inflammatory, T. gondii (Profilin-like)

T. gondii Profilin is a 163 amino acid, 18 kD profilin-like protein that generates a potent interleukin-12 (IL-12) response in murine dendritic cells through binding to Toll-like receptor 11 (TLR11). TLR11 does not appear to be expressed in human. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. The detection limit for T. gondii Profilin is ~0.2ng/well. , Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents detects rat T. gondii Profilin. The detection limit for T. gondii Profilin is ~1ng/lane under non-reducing and reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.