Anti-PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, E

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin. Applications: Suitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:1000, Western Blot:1:500-1:1000, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.