Anti-PHD2 (Prolyl Hydroxylase Domain-containing Protein 2, C1orf12, HIFPH2, HPH2, SM20, ZMYND6, EGLN

PHD2 (Prolyl Hydroxylase Domain-containing protein 2, also HPH2, EGLN1 and HIF-PH2) is a 45-47kD dioxygenase member of the PH family of enzymes. It is ubiquitously expressed, and serves to regulate the availability of the oxygen-sensitive HIF transcription factor. Active HIF1alpha is a heterodimer of alpha- and beta-subunits and when intact, promotes VEGF and EPO production. The beta-subunit is constitutively expressed, while alpha-subunit levels are regulated by intracellular oxygen concentration. At normoxic levels, the alpha-subunit is hydroxylated on Pro by one of three PHDs, inducing its ubiquitination/degradation. The hydroxylation event requires oxygen, and thus PH activity (particularly PHD2) is a measure of a cell's oxygen concentration. Human PHD2 is 426 amino acids (aa) in length. It contains an NES (aa6-20), a Zn-finger region (aa21-58), and a catalytic domain (aa291-392). There are five nitrosylated cysteines plus one acetylated alanine. Two isoform variants are known, one that shows a deletion of aa338-359, and another that contains a 17aa substitution for aa58-175. Over aa157-426, human PHD2 shares 93% aa sequence identity with mouse PHD2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.