Anti-PAK2 (p21 Activated Kinase 2)

Anti-PAK2 (p21 Activated Kinase 2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P2488-12H.20 20 µl - -

4 - 22 business days

330.00€
P2488-12H.100 100 µl - -

4 - 22 business days

566.00€
 
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular... more
Product information "Anti-PAK2 (p21 Activated Kinase 2)"
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation of Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). More recently identified family members including PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation of Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: P2488-12H

Properties

Application: WB
Antibody Type: Monoclonal
Clone: 8A25(C17A10)
Host: Rabbit
Reactivity: Human, Monkey, Mouse
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the amino-terminal region of human PAK2.

Handling & Safety

Storage: -20°C
Shipping: °C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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